• Novita Sari Barus
  • Sony Sony STIKes Nurliana Medan
  • Salman Salman Universitas Tjut Nyak Dhien
  • Mahmudi Mahmudi
  • Rita Sunartaty Universitas Serambi Mekkah
Keywords: Uji Toksisitas, Daun Tembakau, Fermentasi


Penilitian ini mempunyai tujuan jangka panjang yang ingin di capai adalah mengetahui toksisitas dari tanaman tembakau yang terfermentasi yang diaplikasikan pada mencit, dan melihat histologi dari organ vital pada mencit. Target  khusus  yang  ingin  dicapai  dalam  penelitian  ini  adalah  Mengetahui toksisitas sub akut daun tembakau yang terfermentasi yang diaplikasikan pada mencit, dilihat melalui histologi organ vital mencit, Mengetahui dosis toksin yang ditimbulkan daun tembakau yang terfermentasi untuk kesehatan masyarakat. Metode  yang  akan  di  pakai  untuk  mencapai  tujuan  jangka  panjang  dalam penelitian ini menggunakan penelitian secara eksperimen dengan ketentuan- ketentuan menentukan sampel Mencit (Mus Musculus) , Pengambilan sampel Mencit sebanyak 36 ekor berdasarkan berat badan, jenis kelamin., Pemberian toksin daun tembakau yang terfermentasi terhadap mencit, Pemeriksaan uji toksisitas sub akut terhadap mencit dan melihat histologi organ vita pada mencit.


Alworth, W. L., & Rapoport, H. (1965). Biosynthesis of the nicotine alkaloids in Nicotiana glutinosa: Interrelationships among nicotine, nornicotine, anabasine, and anatabine. Archives of biochemistry and biophysics, 112(1), 45-53.
Arsad SS, Esa NM, Hamzah H. 2014. Histopathologic change in liver and kidney tissues from male Sprague Dawley rats treated with Rhaphidophora Decursiva (Roxb.) schott extract. J Cytol Histol. (S4):001.
Bakht, J. A. and Shafi, M. (2012). Antimicrobial activity of Nicotiana tabacum using different solvents extracts. PakJ Bot; 44:459-463.
Burton, H. R., Dye, N. K., Bush, L. P. Distribution of tobacco constituents in tobacco leaf tissue. 1. Tobacco-specific nitrosamines, nitrate, nitrite, and alkaloids. J. Agric. Food Chem. 1992, 40, 1050-1055.
Charles Scerri, (2005). Nicotine : Pharmacology and Therapeutic Implications in Neurodegenerative and Psychiatric Disoders, Maltamedical journal,I(4),17-21.
Christakopoulos, A.; Feldhusen, K., Norin, H.; Palmqvist, A., Wahlberg, I.
Determination of natural levels of coumarin in different types of tobacco using a mass fragmentographic method. J. Agric. Food Chem. 1992, 40,1358–1361.
Dewey, L. J., Byerrum, R. U., & Ball, C. D. (1955).The biosynthesis of the pyrrolidine ring of nicotine. Biochimica et biophysica acta, 18, 141-142.
Emordi, J. E., Agbaje, Oreagba, Iribhogbe, & Ota, (2015). Evaluation of hypoglycemic activities of hydroethanolic leaf extract of Nicotiana tabacum. International Journal of Herbs and Pharmacological Research, 4(2), 2-9.
Harborne JB. 1987. Metode Fitokimia. Edisi ke-2. Padmawinata K, Soediro I, penerjemah. Bandung (ID): Institut Teknologi Bandung. Terjemahan dari: Phytochemical Methods.
Hinds, J. I. D. The use of tobacco; Cumberland Presbyterian Publishing House: Nashville, TN, 1882; pp 1-138.
Kiernan JA. 1990. Histological & Histochemical Methods. Theory and Practice. Ed. Ke-2. Oxford (UK): Pergamon Press.
Ksir, C. and Benson, D.M. (1983). Enhanced behavioral response to nicotine in an animal model of Alzheimer's disease. Psychopharmacology; 81:272-273.
Lee, J.E., Kim, H.J., Choi E.K., Chai, H.Y., Yun,Y.W., Kim, D.J., Nam, S.Y., Lee, B.J. Ahn, B.W., Kang, H.G., and Kim, Y.B., (2003).Fourweek repeated-dose toxicity study on Pinellia Extract. Korean J. Lab. Anim. Sci.19, 127–141.
Mizusaki, S., Kisaki, T., & Tamaki, E. (1968). Phytochemical studies on the tobacco alkaloids. XII. Identification of γ-methylaminobutyraldehyde and its precursor role in nicotine biosynthesis. Plant physiology, 43(1), 93-98.
OECD (2001). OECD Guidelines for the Testing of Chemicals, No 420. Acute oral toxicity- Fixed Dose Procedure. Paris, France: Organization for Economic Cooperation and Development.
Parasuraman, (2011). Toxicological sreening. J. Pharmacol Pharmacother, 2(2),74-79.
Philip Morris International, 2016, Reduced-risk product scientific update,https://www.pmi.com/resources/docs/default-source/pmi- innovation/pmi-scientific-update-sept-2016.pdf?sfvrsn=2457b6b5_2
Rawat, A., & Roshan Mali, R., (2013). Phytochemical Properties and Pharmcological Activities of Nicotiana Tabacum: A Review, Indian Journal Of Pharmaceutical & Biological Research (ijpbr), 1(1), 74-82.
Ren Nan and Timko Michael., (2001). AFLP analysis of genetic polymorphism and evolutionary relationship among cultivated and wild Nicotiana species,44 (4), 559-571.
Sela-Buurlage, B. M., Ponstein, S.A., Bres-Vloemans, A.S., Melchers, S.L., van den Elzen, J. M. P. and Cornelissen J.C. B. (1993). Only Specific Tobacco (Nicotiana tabacum) Chitinases and 8-1,3-Clucanases Exhibit antifungal activity. Plant Physiol, 101, 857-863.
Tricker, A. R., Ditrich, C., & Preussmann, R. (1991). N-Nitroso compounds in cigarette tobacco and their occurrence in mainstream tobacco smoke.
Walum, E. (1998). Acute oral toxicity. Environmental health perspectives; 106 (2):498.
Whitehouse, P.J., Martino, A.M., Antuono, P.G., Lowenstein, P.R., Coyle, J.T., Price, D.L. et al. (1986). Nicotinic acetylcholine binding in Alzheimer's disease. Brain Res; 371:146-151.
Wilson, A.L., Langley, L.K., Monley, J., Bauer, T., Rottunda, S., McFalls, E. et al (1995). Nicotine patches in Alzheimer's disease: pilot study on learning, memory, and safety. Pharmacol Biochem Behav, 51, 509-514.
Wong, H. L., Murphy, S. E., Wang, M., & Hecht, S. S. (2003). Comparative metabolism of N-nitrosopiperidine and N-nitrosopyrrolidine by rat liver and esophageal microsomes and cytochrome P4502A3. Carcinogenesis, 24(2), 291-300.